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KMID : 0861020230380060045
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Korea Journal of Herbology
2023 Volume.38 No. 6 p.45 ~ p.52
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Effects of baicalein on hydrogen peroxide productions in mouse macrophages stimulated by lipopolysaccharide and peptidoglycan
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Park Wan-Su
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Abstract
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Objectives : Effects of baicalein (BA) on oxidative stress in RAW 264.7 mouse macrophages stimulated with peptidoglycan (PG) and lipopolysaccharide (LPS) were investigated.
Methods : RAW 264.7 co-stimulated with LPS and PG were incubated with BA at concentrations of 25 and 50 μM. Incubation time was 18 h, 20 h, 22 h, 24 h, and 26 h. After incubation, the production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Additionally, RAW 264.7 stimulated with PG were incubated with BA at concentrations of 25 and 50 μM for 24 h. After incubation, NO production was evaluated by griess reagent assay.
Results : BA significantly inhibited hydrogen peroxide productions (p <0.05). In details, production of hydrogen peroxide in ‘LPS and PG’-stimulated RAW 264.7 treated for 18 h with BA at concentrations of 25 and 50 μM was 91.27% and 89.22% of the control group treated with LPS and PG only, respectively; the production of hydrogen peroxide for 20 h was 92.19% and 90.58%, respectively; production of hydrogen peroxide for 22 h was 91.69% and 89.89%, respectively; production of hydrogen peroxide for 24 h was 92.4% and 90.19%, respectively; production of hydrogen peroxide for 26 h was 91.7% and 89.04%, respectively. Additionally, BA at the concentration of 50 and 100 μM significantly inhibited NO production in PG-induced RAW 264.7 (p <0.05).
Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in ‘LPS and PG’-stimulated RAW 264.7 macrophages.
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KEYWORD
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RAW 264.7, Baicalein, Macrophage, Lipopolysaccharide, Peptidoglycan, Hydrogen peroxide
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